1ϲԱՂ,&&v :}ܺΰ?<))Pt<&/qqqbZh * ct PTyyX e,> ꎥ1 Kţ71:迳.`[Ҹgĸ鱷oժ nĸ0gԻ48cɰF :F xK^jQk:c 0 c \ 1baoǕt Pԝ;wct PQiiK0 O_*x c 8c Z> c @t 1 ^I`q\ @o1 %o5xrr)Ec TXLLx/ hǂ&͝;ЛNj}j *jx @ wc 1 tct 0/c % ʢc %QQoV~t PWYot PZKMx^:(t ZnܸQRq:@-u^p~ ZJJJdBC+PThh؋ccɎޱmcf͚%b_`K:@r
Y۶1(>}: c!c1 F:4bt ct w5JV1 j"_ݺQ&;v9:@}]bbx}&,9@]%~s=gbٱ_~Ev, PWǎ .]J HN鏌 r&N(v{/_ խ;t0h :@E5[C (GB&1 Jwlyt Z._l߱Ç1 ʑKYYYt rƏ /#ֱcG1 l;_v : M혯|z _׃,l ]}ox5c}įe.W} c ѾSUva.ބ066{{hʃ5u?ҸcZ\i ۻ.[ͮcwu|GqkTzﴏ$$$8qbٲe'O YkI#321nU ~xx Ξ=LCK:F\~]߁ar 6߿{_+) kֿO/֭[{{i){ӲcmhyשSD+@W^7mڤʂ cllt[n`Cp֙?~`o:Q?z GLvlyڳ1o ;
i c>%3DdǦ uUDzO >5+O?[AzNO=}n32N/Z*SB 3`?BM8o^>x !ֱe6}ȠAq?h}1juͽ~w df((~FǼz>r'xby* KKK ^5DF饗>/>}hkڹ;qq/| o]?vL622FΝU~v/l F\:-b&MV"5s:ٸwP'O\o^y啋/0`le˖}gn۶͙ 鑑KBj #Uw ~;?#ZrD
T{>"->h'vN}ng֮9,ʲ/ =:( ;hީʳ0ܹsY0/99yƌuo/\
>o/W}W
[<1s~/س)c x_;AA:k߾Ԧ^rE KMMm^dÍ8}嗵+==>h˗/w]jժ WMI9"rKu- L
蘧ֿWR۸qc3:%ZvLd̶SYYŋnٲE V^͆
`w_Hﾛ
n9i$'o}B-5իsjT sQ7lذ/|EHٱ2;MFjJ]ݼyĉ_z饺ON:K,IJJjV~dݺ+\"AZ 5kֈSL=*g~īM{xꫯwSRRCzuz;v;G6l8b?ccO_zUwټ& ZXHH
KUyQFvGJU֯ߧc:X ۺuk3:v! v5j}-ZTt Bf{9R+ߤ/4ڃ -]ee'|"?bŊ&],' 1mǴ3cwL;E657icz߾}bhʕ^ ݸ%_}yydL0u;ٱ酱ΟuMP:eLػ
c7[jV J۽{{\p \e@zOȑ:]}i#u̙cZHRSSWZ%'j:dHXDDX||*OF;K7۷o'&&edHMW/s᧺ ɕ3zB6Z?^,cⳜ7''Ӝߝ33O/1L6u,2XƦOn9%',77ޔu5l[YCW_}yjk"Cv)2mSܭ[EZREbx .?eZ/^,ejPLnKֱQ Nl D݅y F<0BJ';vIǧdfΜ< UQf*--uzՀ,\pɒ%ObdvNc;vM SɎ9>E&"+h-[4ٷzK똜YvZs7l8C+Zۊ6KJJR&;6pSd[pرcp;wlڴ#{ѣҥKtcY]N7t/ c;w4xצ[[[';ʕ+ׯ__wp*z( u믧c[ķѺukMu^dܞ?4íwpuv7;VKcG1'4 _YYc>C fc [9mk/Y&\k0ֳgOCN䍮 *jcѣG
1m
f]t G }T XMo o!;&o-20PEDDֺ@k)[jx Ǝ +S̩{7|y :eMgӦ/ڱ۷cw,nϓ !^+~c={^ ;s:6f̘+ ?~'6NLIK߄ǚ1A{ѢE\ ;vynjtQ Jسfw,6vwݎ1 -Aֽfw,(h,X裏j 3I~:˗[1y5k|}}
c:&Wڴi +eǴbV2u1u,//?(:ٳu sŔeee1 ^Xˋڷoo 1⍎tPc[c q% ܱfϻc 1 zq~ c {\I xQ Ӗ菈(a ۵k Eqqqb?Z65bŲcΝc (--M.ԎM|Y@@Dt й
ٱ+\$N 5{ 2:@'}8 XS1bt 0|Oǚw\x 1:1䚽DIc;&/J:v qIcB㱦2xy 3"t ct ǎ+w~:V!! Us+tz駛u+^^SNP@ HHHhv8x`t P9s2o&) ݮ];:@'z8 7MRvm۶t c 8c L{\I 1: |P\_ʢc 3~x ;?PUU Eeggڵkt 1 hjzU )~XVּ/}8E^
\
50ȗ3 @ @
> Goal Cell E Concentration of A (M) Concentration of B (M) Starting Length of plug A (cm) Starting Length of plug B (cm)% Effective length of the capilary (cm) Migration time of A (s) Migration time of B (s)
1. A single ppKCE electropherogram can be used to find the following parameters: C, Adis, Afree, tC, and tA. A separate experiment may be needed to find tB if B is not detectable in the ppKCE electropherogram. U X
[ _
a b c
i j k
Migration time of C (s)x 2. Insert the experimentally determined values and experimentalist-controlled parameters in the yellow cells (C12-C22). Afree Adis C [A] [B] lA lB l tA tB tC Dissociated A (a.u.) Intact comlex (a.u.) kon koff Noninteracted A (a.u.)? 3 . C l i c k t h e C a l c u l a t e b u t t o n o n t h e s p r e a d s h e e t ( c e l l A 2 3 ) . 4 . T h e v a l u e s f o r k o n a n d k o f f w i l l a p p e a r i n p i n k c e l l s ( c e l l s C 2 5 , a n d C 2 6 ) . T h e a c c u r a c y o f c a l c u l a t i o n s c a n b e m o n i t o r e d t h r o u g h t h e G o a l C e l l v a l u e , w h i c h s h o u l d b e e q u a l t o z e r o a f t e r c a l c u l a t i o n s .
ALL RIGHTS RESERVED " )^ ^ ` &a
@ / mm v y
d MbP?_ * + % M S a m s u n g C L P - 5 0 0 S e r i e s
od X X L e t t e r PRIV 2 2 2 2 2 2 2 2 2 2 X p
2 2 d C o u r i e r N e w H " d XX ? ? U } ! } } /
, @
, @
, @
, @
, @
, @
, @
, @
, @
, @
0
,
0
; < = = = = = = = > 7 5 5 5 5 5 5 5 5 6 7 5 5 5 5 5 5 5 5 6
3 4 5 5 5 5 5 5 5 6 7 5 5 5 5 5 5 5 5 6
3 4 5 5 5 5 5 5 5 6 7 5 5 5 5 5 5 5 5 6
3 4 5 5 5 5 5 5 5 6 7 5 5 5 5 5 5 5 5 6 8 9 9 9 9 9 9 9 9 :
, ~
`@
)
~
@^@
) ~
F@
) H>
) H>
* ~
D@
* ~
D@
* ~
D@
* ~
y@
* ~
@a@
* ~
@j@ ' ! ' !
+ & B " j>FA , D D D D D D
+ & . " kWouy? D D
A D ' !
& o # K 4 Y D D D D
D A D D D D D A A D
$ ( % Dn? - . . . . . / ' ' ' ' ' ! D l* * * * B B B F F B B B B B B v b B
!
"
#
$
%
&
'
(
)
*
+
,
-
.
? ' ' ' ' ' ! ! ' ' ' ' ' ! " ' ' ' ' ' ! # ' ' ' ' ' ! $ ' ' ' ' ' ! % ' ' ' ' ' ! & ' ' ' ' ' ! ' ' ' ' ' ' ! ( ' ' ' ' ' ! ) ' ' ' ' ' ! * ' ' ' ' ' ! + ' ' ' ' ' ! , ' ' ' ' ' ! - 0 1 1 1 1 1 2 " & : # @ b (
^ 0
# v ] ` + p h
0 @ ? ]
` <, p h
0 @ ? ] ` ,- p h
0 @ ? ] ` . p h
0 @ ?
] ` . p h
0 @ ? D \ ] ` / v n
6 A ̙ ? ? 0 ] & ` 0 v n
6 A ̙ ? ? ] & ` 1
B N_
@ ? ]
`N_|2
< Afree< v n
6 @ ? ] ` 2
B O_
@ ? > b ] `O_\4
< Adis< , v n
6 @ ? P P ] ` 4
B `O_
@ ? ] ``O_<6
< C< v n
6 @ ? ] ` 6
B O_
@ ? ] `O_ 8
< tC< v n
6 @ ? ] ` 8
B 1_
@ ? ] `1_9
< tA< % v n
6 @ ? ] ` `: x x
6 (1_ C C @ - ] , @(1_; 2_9
$ <
Calculate< [ l l
s * /_
@ + i P, ] `/_,<
n ^ N @
L
# # 9 - ] # ` < R J
"
3 A N @
L ] & " ` @= v n B
6 D @
] ` > v v
6 P_
@ ; ] ` P_>
< tB< _
> @
" 7
8AB2
M o d u l e 1 _ _ S R P _ 2 L _ _ S R P _ 3 ! g -B0=830 #
" $ % & ' ( ) * + , - . / 0 1 2 4 5 6 7 8 9 : ; < = > ? @ A B D E F G H I J K L M N O P Q R S T U V W X Y Z [ \ ] ^ _ ` a b c d e f g h i j k l n o p q r s t u w x y z { | } ~ C ,Hޑ x ME P 6
L L L L L ( 8 0 % 0 ` x k ` 0 @H l @x \ @ X @ H @ 8 @4 @ $ @ @ @ @= 8 8 ֘D $ * \ R f f f f * 1 ; 4 4 b 6 9 9 1 9 * \ R 1 * # 2 2 9 * \ R 1 * # c 4 * \ R 1 * # 1 7 5 * \ R 1 * # c 4 * \ R 1 * # c 4 * \ R 1 * # c 4 * \ R 1 * # c 4 " . [ 0 h o p 8 @ H P C29 $ C28 $ B@ X r Attribut e VB_Nam e = "Mod ule1"
S ub FindP ar()
Ra nge("C28 ").GoalSeek :=0, Ch >ing@Cell:=X9B" tEnd
rU ~| Y 9 y ` [($ %X'\%:l % $
QXo%4'8%:H
% $
4 45 ` $ X 4 k rU $ ` n ' ,H* # x ME ( SL S S < N 0 { 0 0 0 2 0 8 1 9 - 0 0 0 0 - 0 0 0 0 - C 0 0 0 - 0 0 0 0 0 0 0 0 0 0 4 6 } @ ( % H @ 0 ֘D x Attribut e VB_Nam@e = "? "D
Bas|0 {00020819- 0C A$0046}
|G lobalSpacFalse dCreatabl Predec$la Id Tru
BExpose TemplateDeriv$BustomizD2 ' ,H # x ME 8AB2
3 _ V B A _ P R O J E C T C M
d i r m _ _ S R P _ 0 v ( SL S S < N 0 { 0 0 0 2 0 8 2 0 - 0 0 0 0 - 0 0 0 0 - C 0 0 0 - 0 0 0 0 0 0 0 0 0 0 4 6 } @ ( % H @ 0 ֘D x Attribut e VB_Nam@e = "? 2"
Basp 0{00020820- C A$0046}
|G lobalSpacFalse Creatabl Predec$la Id Tru
BExpose TemplateDeriv$BustomizD2 am * \ G { 0 0 0 2 0 4 E F - 0 0 0 0 - 0 0 0 0 - C 0 0 0 - 0 0 0 0 0 0 0 0 0 0 4 6 } # 4 . 0 # 9 # C : \ P R O G R A ~ 1 \ C O M M O N ~ 1 \ M I C R O S ~ 1 \ V B A \ V B A 6 \ V B E 6 . D L L # V i s u a l B a s i c F o r A p p l i c a t i o n s * \ G { 0 0 0 2 0 8 1 3 - 0 0 0 0 - 0 0 0 0 - C 0 0 0 - 0 0 0 0 0 0 0 0 0 0 4 6 } # 1 . 3 # 0 # C : \ P r o g r a m F i l e s \ M i c r o s o f t O f f i c e \ O f f i c e \ E X C E L 9 . O L B # M i c r o s o f t E x c e l 9 . 0 O b j e c t L i b r a r y * \ G { 0 0 0 2 0 4 3 0 - 0 0 0 0 - 0 0 0 0 - C 0 0 0 - 0 0 0 0 0 0 0 0 0 0 4 6 } # 2 . 0 # 0 # C : \ W I N D O W S \ S y s t e m 3 2 \ s t d o l e 2 . t l b # O L E A u t o m a t i o n
* \ G { 2 D F 8 D 0 4 C - 5 B F A - 1 0 1 B - B D E 5 - 0 0 A A 0 0 4 4 D E 5 2 } # 2 . 1 # 0 # C : \ P r o g r a m F i l e s \ M i c r o s o f t O f f i c e \ O f f i c e \ M S O 9 . D L L # M i c r o s o f t O f f i c e 9 . 0 O b j e c t L i b r a r y
֘D ,H M o d u l e 1 1 ; 4 4 b 6 9 9 1 9 M o d u l e 1 ޑ ! -B0=830 1 6 4 4 b 6 9 8 9 e ) -B0=830* -
8AB2 1 8 4 4 b 6 9 8 9 e -
8AB2 H - 0 h H VVUoE|ғ +ɔT@Hq- Pj mh#PCN5%` ' Excel+ VBA Win16~ Win32 Mac VBA6# Project1
stdole`
VBAProject Officeu Module1b _Evaluate 1 G Range GoalSeekY' GoalI ChangingCell? Application* CommandBars
Visible aj 1 2 3 ( FindPar Worksheet Workbookk ` ) -
6 0* pH d
VBAProject 4 @j
=
r ֘D J<
rstdole> s t d o l e
h %^ *\G{00020430- C
004 6}#2.0#0 #C:\WIND OWS\Syst em32\e2. tlb#OLE Automati on ` EOffDicEO f i cEE 2DF8D04C -5BFA-101B-BDE5EAAC42E gram Fil es\Microsoft 6\ MSO9.DLL#
9.0 Ob Libra,ryC ,H
Module1G d u1 2 HB1m!@h B,Bޑ!EB+B G @-B0 =83kA? 2 - *" T2G§ A 2g:A 22cIA5 K*m rU ~ ~ ~ ~ ~ ~ ~ ~g 9
$p7B)! a p i
Module1 1 2 3
VBAProject F / C:\PROGRA~1\COMMON~1\MICROS~1\VBA\VBA6\VBE6.DLL VBA
Y 0 F
3 C:\Program Files\Microsoft Office\Office\EXCEL9.OLB Excel
Q y @ 0 F C:\WINDOWS\System32\stdole2.tlb stdole C 2 9
Y P L-[ DR
1 C:\Program Files\Microsoft Office\Office\MSO9.DLL Office
a ` FindPar
B 8 F F E 1 F AİG@ % O F !.1VQFM*w7M D F Worksheet
@ B 1 0 B 1 2 B 3 0 B 2 9 C 2 8 { rU ~}
p Module1 M o d u l e 1 -B0=830 2 8AB_ _ S R P _ 1 n P R O J E C T w m G P R O J E C T
S u m m a r y I n f o r m a t i o n ( 2 ID="{5EB1D709-DE9C-4B63-AAC7-55639A21EFA8}"
Module=Module1
Document=/&H00000000
Document=2/&H00000000
Name="VBAProject"
HelpContextID="0"
VersionCompatible32="393222000"
CMG="5C5E4DC1556B596B596B596B59"
DPB="0A081BB7C8B8C8B8C8"
GC="B8BAA9CAAACAAA35"
[Host Extender Info]
&H00000001={3832D640-CF90-11CF-8E43-00A0C911005A};VBE;&H00000000
[Workspace]
Module1=66, 87, 750, 567, Z
=0, 0, 0, 0, C
2=0, 0, 0, 0, C
Oh +'0 @ H X p
victor Sergey Krylov x Microsoft Excel @ (b@ ;% ՜. +,0 P X p x
91ɫ D o c u m e n t S u m m a r y I n f o r m a t i o n 8 C o m p O b j f
Calculations Worksheets
F Microsoft Excel Worksheet Biff8 Excel.Sheet.8 9q